Plasmid

Part:BBa_I745000:Design

Designed by: Weiss Lab   Group: iGEM07_Princeton   (2007-10-26)


Backbone TRE plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1488
    Illegal NheI site found at 1507
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal NotI site found at 85
    Illegal NotI site found at 2865
    Illegal NotI site found at 8876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1488
    Illegal BglII site found at 3630
    Illegal BglII site found at 3696
    Illegal BglII site found at 3737
    Illegal BglII site found at 7929
    Illegal BglII site found at 8934
    Illegal BamHI site found at 1546
    Illegal XhoI site found at 8883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal NgoMIV site found at 4325
    Illegal NgoMIV site found at 5358
    Illegal NgoMIV site found at 5419
    Illegal NgoMIV site found at 5533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3719
    Illegal BsaI site found at 8916
    Illegal BsaI.rc site found at 3479
    Illegal BsaI.rc site found at 7061
    Illegal BsaI.rc site found at 8799
    Illegal SapI site found at 5978
    Illegal SapI.rc site found at 49


Design Notes

We wanted to create a vector that could be modular and one that would allow one to use any promoter, any gene of interest and any selection/fluorescent marker. We designed the promoter with flanking PacI and EcoRI sites and the gene site with SfiI, EcoRI, BamHI and NheI sites. However, due to the lack of available sites in a lentivector, we had difficulty designing a modular IRES-indicator/selection fragment. Work is underway to correct this.


Source

This plasmid was designed by the Weiss lab for use as a lentiviral vector.

References